Table 1.

SoftMax Pro software settings

Settings options				Data reduction
Fluorescence				Wavelength combination: !LM1/!LM2
Bottom read				Kinetic reduction: peak
	Ex	Em	Cutoff*	Smoothing: moving average
Lm1	340	510	495	(Number of points) × 3
Lm2	380	510	495	Limits
Time: 70 s				Min RFU: 0
Interval: 4.22 s				Max RFU: 35,000
Reads: 17				Lag time: 0
PMT: high				End time: 70
Reads/well: 8				Baseline options:
Compound transfer				Zero baseline: 1 point
T1: 130, 2, 50 @ 16
Automix: Off
Calibrate: On
AutoRead: Off