Fig. 2.

Deletion of the SUR4 and VPS21 genes results in synthetic growth defect. (A) BY4741 (wild-type), 1865 (vps21Δ), 5281 (sur4Δ), MRY91 (sur4Δ vps21Δ), 5763 (fen1Δ), and MRY90 (fen1Δ vps21Δ) cells were grown to stationary phase, serially diluted at 1:10, spotted on YPD plates, grown for indicated time at 30°C, 36°C, or 39°C, and photographed against a dark background. (B) Total membrane fractions (20 μg) prepared from BY4741 (wild-type), 1865 (vps21Δ), 5281 (sur4Δ), MRY91 (sur4Δ vps21Δ), 5763 (fen1Δ), and MRY90 (fen1Δ vps21Δ) cells grown at 30°C were incubated with 20 μM palmitoyl-CoA, 73 μM malonyl-CoA, and 27.3 μM [¹⁴C]malonyl-CoA (0.075 μCi) for 1 h at 37°C. Lipids were saponified, acidified, extracted, converted to methyl ester forms, separated by reverse-phase TLC, and detected by autoradiography.