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. 2013 Apr 5;8(4):e60760. doi: 10.1371/journal.pone.0060760

Figure 1. Limnodynastes peronii embryos and embryonic cells.

Figure 1

(A) Late gastrula (Gosner Stage 12). (B) Neurula (Gosner Stage 15). (C) L. peronii embryos continuing normal development 24 hours after removal from holding at 8°C. (D) Post thaw viable and non-viable neurula cells determined with trypan blue live (L) - dead (D) staining; 400× magnification.