Figure 2. Mean viability of cells from gastrulae and neurulae dissociated in Ca²⁺-free SAR/EDTA and incubated in various media for 24 hours.

(A) gastrulae; (B) neurulae. Ca²⁺-free-SAR/EDTA = Ca²⁺-free-SAR containing 4 mM EDTA; SAR = Simplified Amphibian Ringer; CM = culture medium; CM_cent = centrifuged and resuspended in CM. Each mean, n = 6 (2 embryos from each of 3 spawns); error bar = pooled standard error.