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(A–D) gastrulae; (E–H) neurulae. CM = culture medium; SAR = Simplified Amphibian Ringer; dmso = DMSO; glyc = glycerol; numbers 5–20 indicate % v/v concentration of cryoprotectant in media; control = 0% v/v cryoprotectant. Each mean, n = 6 (2 embryos from each of 3 spawns); error bar = pooled standard error.
