Table 1. Recovery of gastrula cells after cryopreservation.

		Time (hours)
		0.5		1.5		2.5		24
Treatment	[CP] %v/v		# Viable		# Viable		# Viable		# Viable
CM control	0	0.0	0/5	0.0	0/5	0.0	0/5	0.0	0/5
CM/DMSO	5	0.0	0/6	0.6	1/6	1.4	1/6	0.0	0/6
	10	11.1	2/6	0.0	0/6	3.7	1/6	0.0	0/6
	15	8.9	4/6	0.5	1/6	3.1	2/6	0.0	0/6
	20	1.6	1/6	0.7	1/6	0.0	0/6	0.0	0/7
CM/Glycerol	5	1.7	1/6	0.0	0/6	1.2	2/6	0.0	0/6
	10	5.2	2/5	2.6	2/6	0.0	0/6	0.0	0/6
	15	6.4	3/5	0.0	0/6	1.5	1/6	0.0	0/6
	20	9.8	4/6	1.9	2/6	1.5	2/6	0.0	0/6
SAR control	0	0.0	0/6	0.0	0/6	0.0	0/6	0.0	0/6
SAR/DMSO	5	3.5	2/6	0.0	0/6	0.0	0/6	0.0	0/5
	10	8.3	3/6	1.7	1/6	0.0	0/6	0.0	0/6
	15	1.4	1/6	1.8	1/6	0.0	0/6	0.0	0/6
	20	0.0	0/6	0.0	0/6	0.0	0/6	0.0	0/6
SAR/Glycerol	5	5.9	2/6	2.1	2/6	3.5	2/6	0.0	0/6
	10	9.9	5/6	3.9	4/6	4.3	3/6	0.0	0/6
	15	2.7	1/6	5.0	1/6	4.4	3/6	0.0	0/6
	20	6.5	2/6	8.0	2/6	7.4	2/6	0.0	0/6

Post-thaw recovery of cell viability (live/dead staining) expressed as mean number of viable cells per thousand counted (left hand column at each time) and the number of replicates with viable cells (right hand column at each time) for each treatment group. [CP] = cryoprotectant concentration.