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. 2013 Apr 5;8(4):e60657. doi: 10.1371/journal.pone.0060657

Figure 1. Growth of H. pylori in the presence of increasing concentrations of DHA.

Figure 1

A) DHA was added to the H. pylori culture strains 26695, SS1 and B128 after 12 h of growth. Each experiment, consisting of a control (non-treated H. pylori culture) and H. pylori incubated with DHA (concentrations of 50 µM, 100 µM, 250 µM and 500 µM) performed in triplicate. Ethanol (0.06% v/v) the solvent of DHA stock solutions was also tested in these experiments and it did not influence H. pylori growth. B) H. pylori was grown in liquid cultures with concentrations of DHA ranging from 50 µM to 1000 µM. Twenty-four hours later cells were pellet and put to grow into a fresh medium without DHA. H. pylori growth was irreversibly inhibited by DHA at the highest concentrations (≥500 µM of DHA). Data are expressed as the mean ± Standard Deviation and are representative of three independent experiments. * Refers to significant differences in H. pylori growth between controls and DHA-treated conditions (50 µM to 1000 µM of DHA).