Figure 3. Secretions from NF-κB activated PCa cells induce osteoclast-like cell formation.

(A) Conditioned medium from NF-κB activated PCa cells induces osteoclast-like cell formation in vitro. Bone marrow-derived macrophages were treated with conditioned media from LNCaP, NF-κB activated LNCaP-EE, C4-2B and PC3 cells. TRAP staining was performed after 10 days of additional culture with conditioned media. Arrows indicate the osteoclast-like cells. (B) Activation of NF-κB signaling in PCa cells has no significant effect on the osteoblasts proliferation. Primary cultured osteoblasts were treated with conditioned media from NF-κB activated (LNCaP-EE, C4-2B-EV and PC3-EV) or inactivated (LNCaP-EV, C4-2B-KD and PC3-KD) PCa cells. Proliferation assay (MTT assay) was performed at 48 hours after treatment.