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. 2013 Apr 5;8(4):e50068. doi: 10.1371/journal.pone.0050068

Figure 3. CD25 surface expression by flow cytometry in FITC-labeled Scr-Vec-GFP(−) and Scr-Vec-GFP(+) cells.

Figure 3

CD25 surface expression was measured by flow cytometry. T cells from three donors were transfected with the Scr-Vec-GFP(−) directly labeled with FITC (black bars) or Scr-Vec-GFP(+) (white bars). Following 20 hours CD3/CD28 activation cells were stained for flow cytometry analysis. Cells were gated on the total live T cell population by forward and side-scatter analysis, followed by gating on either FITC+ or GFP+ cells, then CD4+ (A) or CD8+ (B) populations were analyzed for CD25 expression and compared by Student's paired t-test. Mean and SEM are shown and P≤0.05 was considered to be significant.