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. 2013 Apr 5;8(4):e60005. doi: 10.1371/journal.pone.0060005

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© 2013 Liang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Titer of serum HBsAg in C57BL/6 or BALB/c mice after HBV plasmid injection. The detection limitation is 0.05 IU/ml. B. Serum HBV DNA levels in C57BL/6 or BALB/c mice after HBV plasmid injection. The detection limitation is 100 copies/ml. C. Bioluminescence image of C57BL/6 (H-2b) or BALB/c (H-2d) mice after hydrodynamic injection. D. Fluc expression in the liver and both serum HBsAg and HBV DNA levels showed strong linear correlation. E. Immunohistochemical staining for HBcAg in hepatocytes of HBsAg-positive C57BL/6 mice (magnification, 200x). In C57BL/6 mice, HBcAg-positive cells were maintained at stable levels on day 3 and day 135. F. Positive rate of serum HBsAg in C57BL/6 (n = 10) or BALB/c (n = 9) mice receiving injection with pGL3/Fluc-HBV1.2 at different time points after injection.