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. 2013 Apr 5;8(4):e61300. doi: 10.1371/journal.pone.0061300

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© 2013 Taylor et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Ply cytotoxicity on HCECs was measured by staining with calcein AM, a selective marker for healthy cells with intact membranes. Percent survival was normalized using PBS (negative) and TX100 (positive) treated cells as controls. The results are shown as % survival. Each mutant was statistically compared to Ply^WT and significant differences (P<0.05) are denoted as a single asterisk (open triangles), 2 asterisks (closed triangles), or 3 asterisks (open diamond) to account for each represented mutant. Error bars are not pictured to prevent obstruction of the data points for viewing.