Figure 7. Oral vaccination via A.C.NPs-legumain induces autoimmune CTLs that target legumain.

Activated CTLs were accessed by flow cytometry. (A) Representative confocal microscopy images of immnofluorescence staining of 4T1 cells treated with or without CoCl₂ for 6 h. E-cadherin was used as a cell membrane marker. Significant translocation expression of legumain to the membrane after CoCl₂ stimulation was observed. (B) Representative flow cytometry histogram of splenocytes co-cultured with 4T1 cells treated with or without CoCl₂. (C) Histogram of the percentages of CD8 and CD25 double positive cells. Data are presented as mean ± SD of five independent experiments (*P<0.01).