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. 2013 Apr 5;8(4):e60573. doi: 10.1371/journal.pone.0060573

Figure 2. The UV-visible spectrum of the purified recombinant CotA.

Figure 2

Optical absorbance spectroscopy of purified CotA was recorded on a DU 800 UV-Vis spectrophotometer. Pure CotA (∼33 µM) in 20 mM Tris-HCl buffer (pH 8.0) was scanned in 1 cm quartz cuvettes at room temperature.