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E. coli strains M15-pQE-cotA (▪) (black square) and M15 (•) (red circle) were grown at 37°C in K liquid medium containing HEPES (pH 7.5), 0.5 mM IPTG, 0.25 mM CuCl2 and 5 mM MnCl2 for 7 days. The values were means ± standard deviations for triplicate assays.
