Table 2. Effects of organic compounds on the Mn(II)-oxidizing activity of purified CotA.
Reagents | Concentration | Relative activity |
CuCl2 | 1.0 mM | 100% |
EDTA | 1.0 mM | 2.36±0.10% |
DTT | 1.0 mM | 54.75±2.51% |
guanidine-HCl | 1.0 mM | 87.30±4.59% |
SDS | 1.0% | 38.78±1.41% |
o-phenanthroline | 0.1 mM | 2.71±0.06% |
The values were means ± standard deviations for triplicate assays.