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. Author manuscript; available in PMC: 2014 Jan 30.
Published in final edited form as: Int J Pharm. 2012 Dec 20;441(0):40–49. doi: 10.1016/j.ijpharm.2012.12.011

Table 2.

Kinetic parameters for Gly-Sar uptake mediated by apical or basolateral peptide transporters in various MDCKII cell lines.

Apical uptake
Basolateral uptake
MDCKII-wt MDCKII-MDR1 MDCKII-MRP2 MDCKII-BCRP MDCKII-wt MDCKII-MDR1 MDCKII-MRP2 MDCKII-BCRP
Km (μM) 59.69 ± 1.10 29.13 ± 3.29 84.75 ± 18.59 60.67 ± 8.56 13.71 ± 2.22 50.87 ± 7.86 64.56 ± 4.09 46.15 ± 2.26
Vmax (fmol/mg protein/min) 10.76 ± 1.68 1.30 ± 0.36 4.62 ± 0.76 4.50 ± 0.83 4.45 ± 0.04 8.52 ± 0.81 8.03 ± 1.78 13.07 ± 2.61
Vmax /Km (nL/mg protein/min) 0.180 ± 0.009 0.045 ± 0.010** 0.055 ± 0.011** 0.074 ± 0.016** 0.324 ± 0.050 0.167 ± 0.058 0.124 ± 0.016 0.283 ± 0.098

Uptake of [3H]Gly-Sar were conducted in the presence of different concentrations of unlabeled Gly-Sar (0–250 μM) at 4 °C and 37 °C, respectively. Each value represents mean ± SD (n = 3).

**

P < 0.01 compared with apical uptake observed in MDCKII-wt cells.