Table 2.

Genes selected from microarray data for validation by qPCR

Protein ID	Sequence	Gene homology
10799	s CGACCGGCCAGGAGAGCAC	regulation of pH - Na/H exchanger
	as GGCTTGAACACGGGCTTGG
27509	s CCCAGAACTACCGCCTTGAA	CVNH domain-containing protein
	as TCAACCCAGCTACCGTCCAC
28147	s GCAAGAACATCCGCGACGAG	C-type lectin
	as CGCATAGCCTTCCTGAGCAT
28324	s GCCAACGAAGAACGAAGCAG	Hypothetical
	as CTGATCGCCCTGTCCCATAA
34827	s CCGAGTCTCTTGTTGCTGTCCTA	ATPase
	as TCGGAGCAGATGTTGGTGAC
56294	s CCGCAGGTGAGAATCAGAGAGT	ATPase
	as CGTCTTCGTCTGTCGGTGTG
68068	s TGAGGATCACTGGCATTGCTC	predicted small secreted cysteine-rich protein
	as GGTAGGTGGGAGATGGCACA
74915	s TTCGTCATGGAGGCTGCTG	ATPase
	as ACCGCAGATGACACCGAAAT
75759	s AATGATACGATGAACACACGACCT	pH-response regulator protein palC
	as GTGAATCTCACGGCCCGAAG
77023	s TTCCACCTCGGCAAATATGATG	methyltransferase
	as TCCTGGCCGTATCAATGAAGA
79497	s TCTCGTTGGTGCCTGGATTG	H+/oligopeptide symporter
	as CAGAGGCGACGAGGATAACG
89677	s GCGCTGATACCTACTGCCTTGA	Fe2+/Zn2+ regulated transporter
	as ATCGGGATCTGCAGTGTCGT
40391	s ACGCCGAGACGCTCTATGAAC	Transcription factor PacC
	as GGTGGTGCGACAAGAGTTCC
67662	s GCTGACCTCCGACTGATTGAAG	ATPase, P-type
	as GCCAGTGGCATCATCAAAGA
43392	s CCTCTGTAACCTCGATTCCAACG	peptidylprolyl isomerase A [PPIA] - normalization gene
	as AGCTCTGGCTCCTGGGTAGG
77851	s CGTACTACAGCCGCTACCAGACC	ribosomal protein L5 [RPL5] - normalization gene
	as AGGCGGTACTTGGGAGCATT
88010	s ACTTCAACGAGGCTTCTGGCAAC	Tubulin - normalization gene
	as CGGAACAGCTGGCCAAAGG
91925	s AGGAAGAAGTTGCTGCCCTCGTCATCGACA	Actin - normalization gene
	as CCCATACCGATCATGATACCATGGTGACG

Following microarray analyses, genes were chosen based on fold change and relevance. Protein ID numbers refer to the T. virens v1.0 database.