Table 1.

Detailed list of primary antibodies used in the immunohistochemical study

Specificity	Antigen or cell detected	mAb/pAb	Dilution	Pre-treatment	Source
Anti-human MΦs	Monocytes and MΦs	mAb	1:100	Proteasea	Serotec
		(clone MAC387)
Anti-bovine TNFα	TNFα	pAb	1: 25	TC-microwaveb	Serotec
Anti-human IL-1α	IL-1α	pAb	1:100	Tween-20c	Endogen
Anti-murine iNOS/NOS Type II	iNOS/NOS Type II	pAb	1:100	TC-autoclaved	BD Transduction Lab.
Anti-murine COX-2	COX-2	pAb	1:75	TC-autoclaved	Cayman Chemical
Anti-Human Von Willebrand Factor	Von Willebrand Factor	pAb	1:800	Proteasea	DakoCytomation

MΦs, macrophages; mAb, monoclonal antibody; pAb, polyclonal antibody.

^a Incubation with 0.1% protease type XIV (Sigma–Aldrich Chemie) in 0.01 M PBS, pH 7.2, for 10 min at room temperature.

^b Incubation with 0.1 M citric acid (pH 6), microwave for 5 min at sub-boiling temperature.

^c Incubation with 0.1% Tween 20 (Merck) in 0.01 M PBS, pH 7.2, for 10 min at room temperature.

^d Incubation with 0.1 M citric acid (pH 6) and autoclaved for 30 min at 121°C, 1 atm.