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. 2013 Apr 5;8(4):e60457. doi: 10.1371/journal.pone.0060457

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© 2013 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A and B: APLNR mRNA was analyzed with real time-PCR. The relative amount of APLNR mRNA in the kidney of type 2 diabetic mice (KK mice) was significantly increased by 5.2-fold compared with that of control mice (C57BL/6). The data are expressed as the means±SD (n = 3, *p<0.01 vs. control group). C and D: The immunohistochemical assay of apelin expression in the kidney showed that the amount of glomeruli with apelin positive staining was significantly higher in type 2 diabetic mice (KK mice) compared to that in control mice (C57BL/6 mice). The bar in C indicates 10 µm. The data are expressed as the means±SD (n = 8, *p<0.01 vs. control group).