Figure 3. Adoptive transfer of CD4⁺CD25⁻Nrp1⁺ T cells changes the expression of inflammatory cytokines and immunomolecules.Both the cardiac allografts and blood samples were harvested 7 days after transplantation.

(A–F) The intragraft gene expression of IFN-γ, IL-17, IL-10, TGF-β, Foxp3 and Nrp1 was analyzed by quantitative real-time polymerase chain reaction. (G–J) Serum levels of IFN-γ, IL-17, IL-10, TGF-β were determined by enzyme-linked immunosorbent assay. Results are presented as mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001. SC = syngeneic control, Nrp1 = neuropilin-1, rapa = Rapamycin.