Figure 2.

TauT knockdown reduces intracellular taurine accumulation. (a) Cellular accumulation of taurine (tracer concentration of ³H-taurine +9 μM unlabelled taurine) reaches steady state after 24 h in control cells (closed squares) and cells treated with non-targeting (open squares) or SLC6A6-specific (open circles) siRNA. Duplicate measurements from one placenta. (b) Scatter plot of intracellular taurine accumulation after 24 h. Each point represents the mean of duplicate observations of three placentas (line represents median). NT=non-targeting siRNA, TauT=SLC6A6-specfic siRNA