Figure 1.
In vitro effects of polymorphonuclear neutrophils (PMNs) on the permeability in a blood–brain barrier (BBB) model (hCMEC/D3 cells) under normoxic or oxygen-glucose deprivation (OGD) conditions. Cells were incubated with high-density lipoproteins (HDLs) (0.4 g/L)±PMNs (1 × 106 cells/mL) for 4 hours (n⩾9 for each condition). Changes in permeability were only observed with PMNs in OGD conditions. Results are presented as box plots in which the median is shown (***P<0.0001 comparing DMEM versus OGD PMNs, P<0.0001 comparing OGD PMNs±HDLs).