Figure 5.

Tail-vein injection of 2MeSADP induces Ca²⁺ increases in wild-type control astrocytes after photothrombosis, but not in astrocytes of IP₃R2 KO mice. (A) Confocal images of the Ca²⁺ responses in wild-type control mice loaded with the Ca²⁺ indicator fluo4-AM before RB-induced photothrombosis, and 3 and 10 minutes after RB photothrombosis. Clotted vessel appears in the middle panel (red pixels with label). Ca²⁺ levels (fluo4 fluorescence) are color-coded (blue is lowest concentration, red is highest concentration). The Ca2⁺ levels of three cells (identified by dashed circles) are followed before and after 2MeSADP leakage across the blood–brain barrier. (B) Single-line traces of the Ca²⁺ responses from these three cells are plotted (ΔF/F). (C) Confocal images of Ca²⁺ response in IP₃R2 KO mice before, and 3 and 10 minutes after RB photothrombosis. Imaging and presentation identical to wild-type mice. (D) Single-line traces of representative Ca²⁺ response of the cells identified in image panels.