Figure 4. A: PDGFR-α protein expression transfected with siRNA.

The PDGFR-α protein expression was seen after transfection with PDGFR-α siRNA. SSc fibroblast cultures were left siRNA III or siRNA₃₁₈₈ (siIII), siRNA II or siRNA₂₂₈₄ (siII), siRNA I or siRNA₁₄₉₅ (siI), siRNA negative control (siNC), and untreated fibroblasts (NC). PDGFR-α protein expression was determined by WB. β-actin served to normalize expression of protein. B: Quantification of the data. RGS for PDGFR-α protein expression by cultured fibroblasts from SSc skin lesions left siRNA III or siRNA₃₁₈₈ (siIII), siRNA II or siRNA₂₂₈₄ (siII), siRNA I or siRNA₁₄₉₅ (siI), siRNA negative control (siNC), and untreated fibroblasts (NC). PDGFR-α protein expression was significantly suppressed in fibroblasts transfected with siRNA.