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. 2013 Apr 5;8(4):e61232. doi: 10.1371/journal.pone.0061232

Figure 3. Limits of detection of specific and cross-reactive anti-Ebolavirus sdAb.

Figure 3

A) What appeared to be the most specific sdAb for each virus were used as captor and phage displayed tracer to titrate cognate virus from 1e+4 to 1e-3 pfu/well. Background signal was established with a set 1e+4 pfu/well of Marburgvirus Musoke. Duplicate wells were utilized for each virus dilution and error bars represent the maximum and minimum values. B) The anti-Zaire sdAb E which appeared to be the most cross-reactive clone was used to titrate all of the viruses including negative control Marburg from 1e+4 to 1e-3 pfu/well. Duplicate wells were utilized for each virus dilution with error bars representing maximum and minimum values. C) PCR titration of Trizol extracted dilutions of virus using universal Filovirus primers [57] to yield a 594 bp band with nv being no virus control and nt being no template control. Amount of virus subjected to RT-PCR (e pfu) across top of gel, mk being marker lanes.