Skip to main content
. 2013 Apr 5;8(4):e60842. doi: 10.1371/journal.pone.0060842

Figure 3. The host innate immune response to Klebsiella pneumoniae infection in Nlrp12−/− mice. A).

Figure 3

Body temperature was monitored daily to assess morbidity. K.pneumoniae infection induced a significant decrease in body temperature in both Nlrp12−/− and wild type mice. B) The lungs were lavaged with HBSS and total BALF cellularity was determined. C) No significant differences in were observed in the BALF neutrophil populations between Nlrp12−/− and wild type mice. However, a significant decrease in the number of monocytes (p = 0.042) and lymphocytes (p = 0.032) were observed in the BALF from the Nlrp12−/− mice compared to the wild type. *P<0.05. D) No differences in bacterial burden were found between the BALF and livers of Nlrp12−/− and wild type animals. E) Histopathology revealed a significant amount of alveolar occlusion following K. pneumoniae infection in both Nlrp12−/− and wild type mice. The magnification bar at 10× = 10 µM and 40× = 5 µM. F) Histology scoring confirmed that no significant differences were identified between the Nlrp12−/− and wild type mice. G) A significant increase in TNFα, IL-6 and IL-1β was observed in the BALF from all K. pneumoniae challenged animals. Saline, n = 3; Nlrp12−/−, n = 13; Wild Type, n = 15. Experiments were repeated 3 separate times with a minimum of 7 K. pneumoniae infected Nlrp12−/− and 7 wild type mice in each group. n.s. = not significant; p = 0.0917.