Figure 4. NLRP12 is not protective during Mtb infection.

A) Nlrp12 ^−/− mice infected with Mtb had no difference in survival compared to wild type mice (median survival was 217 days and 220 days, respectively). B–D) Bacterial burden in the lungs, liver, and spleen was comparable between Nlrp12^−/− and wild type mice at all time points assessed. E) Similar amounts of IL-1β were observed in whole lung homogenates from Nlrp12 ^−/− mice compared to wild-type lungs during both early and chronic phases of Mtb infection. F–G. Cytokine measurements of TNFα (F) and IL-6 (G) showed that Nlrp12 ^−/− and wild-type lungs had similar amounts of cytokines at one day post-infection and during chronic Mtb infection. At 14 days post-infection, Nlrp12 ^−/− lungs had reduced cytokines compared to wild-type lungs that neared significance (p = 0.056 and p = 0.06, respectively). Bacteria burden and cytokine measurements at each time point were taken from 3 mice per genotype per time point. Nlrp12^−/−, n = 19; Wild type, n = 17; Experiments were repeated 2 separate times with a minimum of 12 Mtb infected Nlrp12^−/− and wild type mice in each group. At least 3 mice per strain per time-point were used for CFU and cytokine assessments.