Figure 6. BLAST matrix.

An all against all protein comparison was performed using BLAST to define homologs. A BLAST hit is considered significant if 50% of the alignment consists of identical matches and the length of the alignment is 50% of the longest gene. Internal homology (paralogs) is defined as proteins within a genome matching the same 50–50 requirement as for between-proteome comparisons. Self-matches are here ignored. A comparison of 31 Negativicutes genomes was performed on the CMG-biotools system (9 hours). A high resolution figure can be found as supplemental Figure S2.