Figure 7. Core and pan genome using BLAST.

A pan- and core-genome calculation was performed using BLAST. A BLAST cutoff of 50% identity and 50% coverage of the longest gene was used. If two proteins within a genome matched according to the 50/50% cutoff, they were clustered into one protein family. Protein families were extended via single linkage clustering. If a protein family includes proteins from all genomes in the comparison, the family is a core protein family.