a–c, B6 mice were pre-treated with vehicle or MG (50 and 200 mg/kg) prior to seizure induction with pilocarpine (250 mg/kg). n = 19 vehicle, 12 MG (50 mg/kg), 8 MG (200 mg/kg). a, MG pre-treatment increased the latency to reach stage 3. P = 0.0033 by Kruskal-Wallis test. b, MG pre-treatment reduced the time spent in stage 3. P < 0.0001 by Kruskal-Wallis test. c, MG pre-treatment reduced the highest seizure stage reached. P = 0.0015 by Kruskal-Wallis test. d, Seizures were induced in B6 mice with pilocarpine (250 mg/kg); 10 minutes later, mice were treated with vehicle or MG (200 mg/kg). MG treatment reduced time spent in stage 3. n = 6 per group.
Data are mean ± SEM. * P < 0.05, ** P < 0.005 versus vehicle-treated group as determined by Mann-Whitney U tests.