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(A and B) In vivo primer extension analyses of the loop mutants of MazF-ec and MazF-mt3 in BW25113 ΔmazEF (A) and in BW25113 cells (B). (C) In vivo primer extension analyses of the loop mutants of MazF-ec and MazF-mx in wild-type BW25113 cells. A^CA cleavage sites (MazF-ec) are shown as arrows and asterisks to indicate new cleavage sites in loop 2 mutants and as circles in loop 1 mutants.
