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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Proteins. 2013 Feb 25;81(5):874–883. doi: 10.1002/prot.24246

Figure 4. Poly-glycine mutants of MazF-ec.

Figure 4

(A) Amino acid sequences of poly-glycine mutants. White and black boxes indicate β-sheets (S) and α-helices (H), respectively, on the basis of the secondary structure of MazF-ec (PDB ID: 1UB4). (B) Toxicity of the poly-glycine mutants of MazF-ec. Transformants harboring pBAD33 vector, mazF-ec, /loop1-G12, /Loop2-G8, /Loop2-G11, and /loop1-G12+loop2-G8 plasmids were streaked onto M9 plates in the presence or the absence of 0.2% arabinose and then incubated at 37°C. (C) In vivo primer extension analyses of the ompA mRNA from BW25113 ΔmazEF. A^CA cleavage sites (MazF-ec) are indicated by arrows, and asterisks indicate new cleavage sites in the loop 2 poly-glycine mutants.