TABLE I.
Primers used in this study
| Name | Primer sequences (5′ to 3′) | Purpose |
|---|---|---|
| Loop1-mt3 Fw | gttgatttaggcccgccaagtggtagccagccagctaaacgtcgtccaagtgttgtcctg | Cloning |
| Loop1-mt3 Rv | acctgctgcaaatcgaccgaccgatggtgaaccgcccggatttagttgggtttagtctag | Cloning |
| Loop2-mt3 Fw | tgtacaacgtcaaacacagcattagcggccatgccaggaaatgttgttttatccggtcag | Cloning |
| Loop2-mt3 Rv | ttgttgtaaaggaccgtaccggcgattacgacacaaactgcaacatgttccttgtgt | Cloning |
| Loop1-mx Fw | tgatcctgacgattcaggtcccgtgccatcttactctcgtccaagtgttgtcctg | Cloning |
| Loop1-mx Rv | cctgctctcattctaccgtgccctggagaacttagcagtcctagttgggtttagtctag | Cloning |
| Loop2-mx Fw | tgtacaacgtcaaacttacatcgtgcgtccgagggaaatgttgttttatccggtcaggaa | Cloning |
| Loop2-mx Rv | ttttgttgtaaagggagcctgcgtgctacattcaaactgcaacatgttccttgtgt | Cloning |
| Loop1-F1G12 Rv | tcctcctccacctccaccaccacctccacctccaccatcaacccaaatcag | Cloning |
| Loop1-F2G12 Fw | tggaggtggaggaggacgtccagctgttgtc | Cloning |
| Loop2-F2G8 Rv | acctccaccaccacctcctcctcccgttgtacaaggaac | Cloning |
| Loop2-F2G12 Rv | acctccaccaccacctcctcctccacctccacccgttgtacaaggaac | Cloning |
| Loop2-F3 Fw | aggtggtggtggaggtgttgttttatccggt | Cloning |
| R3 | gtttttaccataaacgttgg | Primer extension |