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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Cancer Res. 2013 Jan 22;73(7):2150–2158. doi: 10.1158/0008-5472.CAN-12-1745

Figure 3. E2F1-depleted osteoblast precursors show impaired induction of osteogenic genes.

Figure 3

A. E2F1 expression determined by qRT-PCR at days 0, 4, and 7 post-induction in cells stably expressing an shRNA sequence targeting E2F1 (E2F1_KD), or pRB (pRB_KD), is shown relative to normal parental cells.

B. Alkaline phosphatase activity, monitored colorimetrically in situ shows sharply increased activity by day 7 post-induction in parental cells, while induction is impaired in E2F1 depleted cells.

C. Quantification of expression levels at days 0, 4, and 7 post-induction, as indicated in panel A, shows E2F1 depletion does not affect Rb1 expression, but has a similar effect as pRB depletion on expression of Alpl (alkaline phosphatase) and Bglap (osteocalcin). Expression values were normalized to Gapdh; β-actin is included as an additional control.