. Author manuscript; available in PMC: 2014 Apr 1.

Published in final edited form as: Cancer Prev Res (Phila). 2013 Feb 27;6(4):339–348. doi: 10.1158/1940-6207.CAPR-12-0311

Table 1.

Effects of experimental diet, gender and Esr2 genotype on offspring survival to 10 months of age.

	All offspring		Male offspring		Female offspring
	Any cause	T-ALL^*	Any cause	T-ALL^*	Any cause	T-ALL^*
Offspring stratified by diet group
Sham-initiated
Control	3.1% (2/65)	0.0% (0/65)	0.0% (0/26)	0.0% (0/26)	0.0% (0/39)	0.0% (0/39)
I3C:M	0.0% (0/65)	0.0% (0/65)	0.0% (0/34)	0.0% (0/34)	0.0% (0/31)	0.0% (0/31)
I3C:O	1.6% (1/64)	0.0% (0/64)	0.0% (0/29)	0.0% (0/29)	2.9% (1/35)	0.0% (0/34)
DBC-initiated
Control	56.7% (68/120)^a	30.7% (23/75)^a	60.3% (35/58)^a	39.5% (15/38)^a	53.2% (33/62)^a	21.6% (8/37)^a
I3C:M	20.0% (24/120)^b	10.3% (11/107)^b	19.0% (11/58)^b	9.4% (5/53)^b	21.3% (13/61)^b	11.1% (6/54)^a
I3C:O	45.5% (55/121)^a	29.0% (27/93)^a	53.2% (33/62)^a	34.1% (15/44)^a	37.3% (22/59)^ab	24.5% (12/49)^a
DBC-exposed offspring stratified by gender
Male	44.1% (79/179)^a	25.9% (35/135)^a
Female	37.4% (68/182)^a	18.3% (26/142)^a
*DBC-exposed offspring stratified by Esr2* genotype**
Wild-type	28.2% (24/85)^a	17.6% (13/74)^a	30.2% (13/43)^a	21.1% (8/38)^a	26.2% (11/42)^a	13.9% (5/36)^a
Heterozygous	32.9% (56/170)^a	18.0% (25/139)^a	38.6% (34/88)^a	23.9% (17/71)^a	26.8% (22/82)^a	11.8% (8/68)^a
Null	50.8% (30/59)^ab	29.3% (12/41)^a	54.8% (17/31)^a	30.0% (6/20)^a	46.4% (13/28)^a	28.6% (6/21)^a
DBC-exposed offspring stratified by diet, then genotype
CON diet group
Wild-type	38.7% (12/31)^a	29.6% (8/27)^a	37.5% (6/16)^a	33.3% (5/15)^a	40.0% (6/15)^a	25.0% (3/12)^a
Heterozygous	57.1% (32/56)^a	31.4% (11/35)^a	62.1% (18/29)^a	42.1% (8/19)^a	51.9% (14/27)^a	18.8% (3/16)^a
Null	66.7% (10/15)^a	28.6% (2/7)^a	66.7% (4/6)^a	33.3% (1/3)^a	66.7% (6/9)^a	4.0% (1/4)^a
I3C:M diet group
Wild-type	4.0% (1/25)^a	4.0% (1/25)^a	7.7% (1/13)^a	7.7% (1/13)^a	0.0% (0/12)^a	0.0% (0/12)^a
Heterozygous	10.8% (7/65)^a	4.9% (3/61)^a	23.1% (3/31)^a	6.7% (2/30)^a	11.8% (4/34)^a	3.2% (1/31)^a
Null	41.7% (10/24)^b	30.0% (6/20)^b	38.5% (5/13)^a	20.0% (2/10)^a	45.5% (5/11)^b	40.0% (4/10)^b
DBCI3C:O diet group
Wild-type	37.9% (11/29)^a	18.2% (4/22)^a	42.9% (6/14)^a	20.0% (2/10)^a	33.3% (5/15)^a	16.7% (2/12)^a
Heterozygous	34.7% (17/49)^a	25.6% (11/43)^a	46.4% (13/28)^a	31.8% (7/22)^a	19.0% (4/21)^a	19.0% (4/21)^a
Null	50.0% (10/20)^a	28.6% (4/14)^a	66.7% (8/12)^a	42.9% (3/7)^a	25.0% (2/8)^a	14.3% (1/7)^a

Note: Different superscripts indicate a significant difference within each comparison group (as indicated by row headings). Life tables analyses (Kaplan-Meir method) (SAS Lifetest procedure) of survival in DBC-initiated offspring were performed in a stepwise manner, wherein death due to any cause or to T-ALL were considered separately. Effects of diet, gender or genotype in sham-initiated animals were not considered as so few deaths occurred in these groups. Initial analyses included all data within the DBC exposure group stratified by gender, diet group or genotype. Then, pair-wise analyses for Esr2 genotype within each DBC/diet group were performed. Analyses were run irrespective of different genders, as well as separately for male and female offspring. Log-rank, Šidák-adjusted P values for pair-wise comparisons between indicated data groups are available in Supplementary Table S2. Incidences of mortality or morbidity are reported as percentages (deaths/total).

Animals that died or were euthanized due to a cause unrelated to lymphoma (anemia, other tumors [lung, liver, skin or ovarian] or cause unknown) during the course of the study were excluded from this tally. Thus, these numbers represent mortality specific to T-cell acute lymphoblastic lymphoma/leukemia (T-ALL).