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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Mar 3;20(4):469–476. doi: 10.1038/nsmb.2519

Table 2.

IC50 values for SOCS3 mutants.

Mutation Region IC50mutant/IC50WT*
WT KIR 1.0 +/− 0.3
SOCS1-3 0.1 +/− 0.05
L22F 0.1 +/− 0.03
L22A 1.2 +/− 0.2
K23A 1.4 +/− 0.1
T24A 3.3 +/− 0.5
F25A no inhibition
S26A 1.0 +/− 0.04
S27A 2.5 +/− 0.2
K28A 2.8 +/− 0.7
S29A ESS 2.0 +/− 0.3
E30A 23.0 +/− 2.9
Y31A 6.0 +/− 0.1
Y47F βA 80 +/− 20
A50E 1.1 +/− 0.2
A50K 6.0 +/− 2.0
T52A 10 +/− 2.0
R77A BC loop 1.4 +/− 0.2
F79A no inhibition
F80A no inhibition
*

Errors are SD from three independent experiments.

≠

SOCS1-3 refers to a SOCS1/SOCS3 chimera which binds to JAK2 with 5–10 fold higher affinity30