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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Eur J Neurosci. 2013 Jan 29;37(7):1090–1102. doi: 10.1111/ejn.12119

Figure 2.

Figure 2

Methods for defining the red nucleus and cuneate nucleus. A. Red nucleus. A1. Darkfield image of the red nuclei. The nuclear region was identified by the dark cell bodies. A2. Fluorescence image of the same section as A1. Fluorogold injected into C6 spinal gray matter labeled the magnocellular red nucleus. A3. Contours drawn under darkfield imaging to outline the parvocellular red nucleus in each animal (separate colors) were highly reproducible. Scale bar, 250µm. B. Cuneate nucleus. B1. Darkfield imaging of the caudal medulla is used to verify nucleus boundaries. B2. Kluver-Barrera staining of an adjacent section is used to validate the location for the nucleus (red) from the surrounding white matter (blue). Scale bars, 100µm.