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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Eur J Neurosci. 2013 Jan 29;37(7):1090–1102. doi: 10.1111/ejn.12119

Figure 3.

Figure 3

Stereological methods of assessing axon length and regional distribution. A. BDA-labeled spinal cord cross section at C6. A1. Montage image of the gray matter. Scale bar, 500µm. A2. BDA-labeled axons at 400x. Scale bar, 10µm. A3. Axons intersect (red arrows) a virtual sphere (“space ball”) that appears as a white circle of varying diameters. Scale bar, 10µm. B. A map of interactions between the “space ball” probe and BDA-labeled axons. The sparse ipsilateral side (left) was sampled more intensely than the dense contralateral side (right). C. Using MATLAB, heat maps of axon length within local regions (40µm by 40µm) are created. Note the difference in scaling of the two sides.