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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Pharm Res. 2013 Jan 15;30(5):1240–1251. doi: 10.1007/s11095-012-0963-6

Figure 5.

Figure 5

Comparative stabilities of CA-lys-TFA and taurocholate against choloylglycine hydrolase from Clostridium perfringens. Panel A shows the loss of CA-lys-TFA (initially 2 mM) and the corresponding appearance of deconjugated cholic acid. Panel B shows the loss of taurocholate (initially 2 mM) and the corresponding appearance of deconjugated cholic acid. Each study was performed in triplicate. After 1 h, only 2.4% of taurocholate remained, while 60.4% of CA-lys-TFA remained, suggesting improved stability of the amide bond linking trifluoroacetyl lysine to cholic acid, compared to the natural amino acid conjugate.