Figure 7.
Representative microscopic images of H&E-stained intestinal villi following treatment with vehicle or two doses of CA-lys-TFA. Intestinal villi from vehicle- and low-dose (150 mg/kg) CA-lys-TFA -treated mice show intact surface epithelium at the tips of small intestinal villi. In contrast, in two of three mice treated with high-dose (300 mg/kg) CA-lys-TFA cellular injury at the tips of intestinal villi was observed (lower, right panel). Villus injury in mice treated with high-dose CA-lys-TFA is characterized by loss of nuclei and cytoplasmic vacuolization in a few cells (thick arrow). Confluent necrosis of the epithelial tip lining with early sloughing toward the lumen is noted in adjacent villi marked with thin arrows. Size bars = 100 micrometers.