Fig. 3. Western blotting of tumor tissue lysates to determine (A) expression of Bax, Bcl2, caspase-3, caspase-9, VEGF, Survivin and Vimentin proteins in tumor lysates by western blotting and (B) quantitation of apoptotic protein expression.
Tumor tissue lysates harvested tumor tissues from control-untreated and treated groups were analyzed by western blotting for protein expressions. Lane 1 = control; Lane 2 = Cxb-NLC; Lane 3 = Doc i.v.; Lane 4 = Cxb-NLC + Doc i.v. Protein expression levels (relative to β-actin) were determined. Mean ± SE for three replicate determinations. One-way ANOVA followed by post Tukey test was used for statistical analysis. P<0.01 (*, significantly different from untreated controls; **, significantly different from single treatments).
