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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Cancer Prev Res (Phila). 2012 Dec 28;6(4):309–320. doi: 10.1158/1940-6207.CAPR-12-0368

Figure 5. BCX restores the SIRT1 protein level and the transcription of tumor suppressor genes and decreases the phosphorylation of AKT.

Figure 5

The lung SIRT1 protein levels are shown in (A). Inset: a representative image of the SIRT1 and GAPDH proteins. The lung sirt1 mRNA levels are shown in (B). The quantification of AKT phosphorylation on Ser473 is shown in (C). Inset: a representative image of phosphorylated AKT (pAKT) and total AKT. The mRNA levels of p53 and RAR-β are shown in (D) and (E), respectively. The proteins were detected using Western blotting. The intensity of the bands was quantified using densitometry and normalized to GAPDH or total AKT levels. The mRNA levels were measured using qRT-PCR and were normalized to β-actin mRNA levels. The columns represent the mean ± sem. *p < 0.05. Abbreviations: BCX (10): β-cryptoxanthin at 10 mg/kg diet; BCX (20): β-cryptoxanthin at 20 mg/kg diet; C: control; Nic: nicotine; NNK: 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone; RAR-β: retinoic acid receptor-β, SIRT1: sirtuin 1.