Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Comp Neurol. 2013 Jun 1;521(8):1891–1914. doi: 10.1002/cne.23265

Figure 4. DMH-NPY projections in close apposition with CART neurons in the PVH of lactating rats.

Figure 4

(A) 20x confocal image showing triple label immunostaining for NPY (green), BDA (red), and CART (blue) in the PVH of a lactating rat. The arrow indicates a fiber containing NPY and BDA near a CART cell body. (B) 40x confocal image showing BDA-containing axonal swelling (white arrow) in close apposition to a CART cell (yellow arrowhead). (C) 40x confocal image showing NPY-containing axonal swelling in close apposition to a CART cell. (D) Overlay image for BDA and NPY co-localized fiber in close apposition to a CART cell. (E-G) Stacks of optical slices (3 μm total at 0.5μm increments) of a CART cell body in close contact with BDA and NPY-containing axonal swelling. Z-coordinates are indicated in single optical slices relative to a reference point, z = 0 μm.