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. Author manuscript; available in PMC: 2013 Apr 6.
Published in final edited form as: Cancer Lett. 2007 Oct 18;258(2):268–275. doi: 10.1016/j.canlet.2007.09.003

Fig. 2.

Fig. 2

Elevated PLD activity in T24 and Calu-1 cells is dependent upon Ras. T24 (A) and Calu-1 (B) cells plated at a density of 105 cells per 60 mm plate. 24 hr later the cells were transfected with either control (Con) scrambled siRNA or siRNA specific for H-Ras (A) or K-Ras (B) as indicated. The levels of Ras and actin were then determined by Western blot and the PLD activity was determined 48 hr later. PLD activity, determined as in Fig. 1, in the Ras siRNA samples was normalized to the PLD activity in the controls, which was given a value of 1.00. Error bars represent the standard deviation for two independent experiments. The data showing the levels of Ras and actin was representative of an experiment that was repeated at least two times.