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. Author manuscript; available in PMC: 2013 Apr 6.
Published in final edited form as: Am J Hematol. 2011 Sep 26;86(12):1001–1006. doi: 10.1002/ajh.22167

Figure 2.

Figure 2

Functional activity of circulating Tregs in chronically transfused SCD and TM patients. Proliferation of sorted CD4+CD25− T effector cells cocultured with autologous sorted CD4+CD25hi Tregs at 1:1, 1:2, and 1:4 (Treg: CD4+CD25−) ratios following stimulation using microbeads precoupled with anti-CD2, anti-CD3, and anti-CD28 (Treg Suppression Inspector) is shown as percentages of CD4+CD25− T cell proliferation when cultured alone (see Materials and Methods section). For some samples, we were unable to obtain sufficient numbers of sorted Tregs, and in those cases, the cocultures were performed at only one ratio of Treg: CD4+CD25−. The average percent proliferation values for alloimmunized transfused SCD patients (n = 12) were significantly higher, when compared with that of non-alloimmunized (SCD patients, n = 10 and TM patients, n = 8, both P < 0.05), indicating that the alloimmunized Tregs are less suppressive. The relative proliferation values of race-matched healthy controls (n = 10 for each of the groups) are also shown.