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. Author manuscript; available in PMC: 2013 Apr 7.
Published in final edited form as: Genesis. 2010 Aug;48(8):492–504. doi: 10.1002/dvg.20650

FIG. 6.

FIG. 6

Treatment with the FGFR1-inhibitor, SU5402, impairs cardiac looping. Widefield microscopic analysis (A) of gross morphology of untreated control, DMSO-treated (2.5 μM), or SU5402-treated (50 μM) embryos at Stage 37 reveals that SU5402 treatment induces edema in the middle-ventral region of the embryo (top right panel) or pericardial edema (bottom right panel) in some embryos. Arrows point to regions of edema. B: Lateral view of whole-mount immunohistochemistry for MHC reveals a fully looped three-chambered heart in untreated and DMSO-treated embryos while those treated with SU5402 appear unlooped. Anterior is to the left and dorsal is to the top in all panels. Total number of embryos analyzed for heart morphology were: n, 22 (untreated), n = 34 (DMSO), and n = 35 (SU5402) and were collected from three separate experiments.