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. 2013 Mar 21;2013:634095. doi: 10.1155/2013/634095

Figure 2.

Figure 2

Effect of magnolol on the number of TRAP-positive multinucleated cells (MNCs) and the expression of RANKL and OPG in gingival tissues. (a) The micrographs (upper panel: 100x magnification; lower panel: 200x magnification) represent the histological sections around intermolar region of rats 8 days after ligature placement, and the quantitative analysis of the number of TRAP+ MNCs with red staining along the bony resorptive lacunae (cells/mm) was performed (scale bar = 50 μm) (c). (b) The protein expression of RANKL and OPG was determined by Western blot in dissected tissues, (d) and the relative protein RANKL/OPG ratio was measured. Data are expressed as mean ± SD (n = 5). **P < 0.01, ***P < 0.001 versus control; # P < 0.05, ## P < 0.01 versus ligation group.