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Effect of magnolol on RANKL-induced osteoclast differentiation. RAW 264.7 cells were cultured with the indicated dose of magnolol (2.5–20 μM) in the presence of RANKL (50 ng/mL) for 4 days. The photographs of TRAP-stained osteoclasts (a), the number of TRAP+ MNCs, and the TRAP activity (b) were examined (scale bar = 100 μm). Data are expressed as the mean ± SD (n = 5). *P < 0.05, **P < 0.01, and ***P < 0.001 versus RANKL-treated group alone.
