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. Author manuscript; available in PMC: 2013 Apr 7.
Published in final edited form as: Mol Cancer Ther. 2009 Aug 11;8(8):2478–2489. doi: 10.1158/1535-7163.MCT-08-1132

Figure 3.

Figure 3

Characterizing NDSP as a secreted protein. A. HEK-293T cells were transfected with control (vector) or NDSP-V5 expression plasmids and supernatant was collected 48 hours after transfection. Supernatants were resolved by SDS-PAGE and immunoblotted with anti-V5 monoclonal antibody. B. Supernatants from HEK-293T cells transfected with control or NDSP expression plasmids were resolved by SDS-PAGE under both reducing (sample buffer with β-mercaptoethanol) and non-reducing (sample buffer without β-mercaptoethanol) conditions. Immunoblotting was performed with anti-NDSP-Ab1. C. Supernatant from SH-SY5Y cells without and with 100ng/ml NGF-β treatment was resolved on SDS-PAGE and immunoblotted with anti-NDSP-Ab1. Control media was simply culture media incubated without cells. D. Additional cell lines were treated with 100ng/ml NGF-β and supernatants were resolved on SDS-PAGE and immunoblotted with anti-NDSP-Ab1.