Figure 3. Both VEGF-independent of NO plus VEGF-stimulated NO are required to maintain normal LSEC fenestration.
LSEC cultured with VEGF+L-NAME (top left panel) or DETA-NONOate (NO donor; top right panel) for 2 days lack fenestration, while LSEC cultured with VEGF+L-NAME+DETA-NONOate (bottom left panel) for 2 days demonstrate normal fenestration. Porosity is shown in the bottom right panel. Scale bar, 5μm. * p<0.001versus VEGF control; NS, not significant; n = 3.